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Related Experiment Video

Updated: Mar 5, 2026

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans

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Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques.

Eladio Abreu^1,2, Rebecca M Terns^1,2, Michael P Terns^3,4

¹Department of Biochemistry and Molecular Biology, University of Georgia, A326B Life Sciences Bldg, Athens, GA, 30602, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 22, 2017

Summary

Researchers visualized human telomerase RNA (hTR) localization using microscopy. This study details methods to track hTR, Cajal bodies, and telomeres, crucial for understanding telomere maintenance and genome stability.

Keywords:

Cajal body Fluorescence in situ hybridization Immunofluorescence Telomerase Telomerase RNA Telomerase reverse transcriptase Telomere

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Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization FISH with PNA Probes in Caenorhabditis elegans

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Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein

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Published on: June 12, 2018

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

Human telomerase is a ribonucleoprotein enzyme essential for maintaining telomere length and genome stability.
Telomerase activity is regulated by intracellular trafficking between Cajal bodies and telomeres.
This trafficking is cell cycle-dependent, primarily occurring during S phase.

Purpose of the Study:

To describe fluorescence microscopy methods for visualizing the subcellular localization of human telomerase RNA (hTR).
To correlate hTR localization with Cajal bodies and telomeres in cultured human cells.
To provide methods for cell cycle synchronization and analysis within S phase.

Main Methods:

Fluorescence in situ hybridization (FISH) to detect hTR and telomeric DNA.
Immunofluorescence to detect Cajal bodies and telomere-binding proteins.
Cell synchronization techniques to isolate cells in S phase for analysis.

Main Results:

Established fluorescence microscopy techniques to visualize hTR localization relative to Cajal bodies and telomeres.
Demonstrated the feasibility of studying telomerase trafficking dynamics.
Provided a framework for analyzing cell cycle-regulated telomere maintenance.

Conclusions:

Visualizing hTR localization offers insights into telomerase regulation and function.
The described methods facilitate the study of telomere biology and genome stability.
Understanding telomerase trafficking is key to comprehending its role in cellular processes.