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Characterization of Klebsiella granulomatis pathogenic to silkworm, Bombyx mori L

M K Mohanta1, A K Saha2, D K M A Saleh2

  • 1Department of Zoology, Rajshahi University, Rajshahi, 6205, Bangladesh. mkmohanta_zool@yahoo.com.

3 Biotech
|March 22, 2017
PubMed

Insights

A novel bacterial disease in silkworms, caused by Klebsiella granulomatis, leads to significant economic losses. Low-dose ciprofloxacin effectively treated the disease, offering a potential solution for silk production.

Area of Science:

  • Veterinary Entomology
  • Microbiology
  • Sericulture

Background:

  • Bacterial infections in silkworms significantly impact silk production and cause economic losses.
  • Identifying the causative agents and their characteristics is crucial for disease management.

Purpose of the Study:

  • To isolate and identify bacteria responsible for silkworm disease.
  • To determine the pathogenicity and antibiotic resistance profile of the isolated bacteria.
  • To evaluate the efficacy of antibiotics in treating the disease.

Main Methods:

  • Isolation of bacteria from diseased silkworm haemolymph.
  • Identification using biochemical tests and 16S rRNA gene sequencing.
  • Pathogenicity testing through re-injection into healthy silkworms.
  • Antibiotic susceptibility testing.

Main Results:

  • Klebsiella granulomatis was isolated and identified as the causative agent.
  • The optimal growth conditions for K. granulomatis were pH 7.0 and 37°C.
  • The isolate exhibited resistance to most tested antibiotics, except azithromycin, gentamycin, and ciprofloxacin.
  • Re-infection caused flacherie-like symptoms and high mortality in healthy silkworms.
  • Low-dose ciprofloxacin treatment was effective in preventing disease.

Conclusions:

  • Klebsiella granulomatis is a pathogenic bacterium causing significant mortality in silkworms.
  • Ciprofloxacin shows promise as an effective treatment for this bacterial disease in sericulture.
Keywords:
AntibioticHaemolymphKlebsiella pneumoniaePathogenic bacteriaSilkworm

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