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Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy
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Two-Color Total Internal Reflection Fluorescence Microscopy of Exocytosis in Endocrine Cells
Adam J Trexler1, Justin W Taraska2
1Laboratory of Molecular Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Methods in Molecular Biology (Clifton, N.J.)
|March 22, 2017
Abstract:
We describe a comprehensive method for imaging and analysis of local protein dynamics at single sites of exocytosis in living cultured endocrine cells. This method is well suited to quantitatively map the complex dynamics of individual molecules at single sites of vesicle fusion in live cells.

