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Related Experiment Video

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Rapid Analysis and Exploration of Fluorescence Microscopy Images
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Fluorescence-Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening.

Manuel Gunkel1, Jan Philipp Eberle2, Holger Erfle2

  • 1BioQuant Center, ViroQuant-CellNetworks RNAi Screening Facility, University of Heidelberg, Im Neuenheimer Feld 267, 69120, Heidelberg, Germany. manuel.gunkel@bioquant.uni-heidelberg.de.

Methods in Molecular Biology (Clifton, N.J.)
|March 22, 2017
PubMed
Summary

We developed multiscale targeted imaging routines to save time and data in fluorescence imaging screens. This method provides broad overviews and detailed views of specific targets identified during screening.

Keywords:
Automated imagingHigh content screeningHigh-throughput screeningKNIMETargeted imaging

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Area of Science:

  • Biomedical imaging
  • High-content screening

Background:

  • Choosing the correct imaging acquisition method is crucial for effective fluorescence imaging-based screening.
  • Appropriate scale selection and readout are essential for successful screening outcomes.

Purpose of the Study:

  • To develop and implement multiscale targeted imaging routines.
  • To optimize imaging time and data storage in screening workflows.
  • To enable on-the-fly identification and acquisition of targets of interest.

Main Methods:

  • Development of advanced routines for multiscale targeted imaging.
  • Integration of image acquisition and image analysis for real-time target identification.
  • Implementation of a system for acquiring both broad overviews and in-depth target information.

Main Results:

  • Successful implementation of multiscale targeted imaging routines.
  • Significant reduction in imaging time and data volume.
  • Effective identification and acquisition of targets of interest during screening.

Conclusions:

  • Multiscale targeted imaging is a valuable approach for optimizing fluorescence imaging-based screens.
  • The developed routines enhance efficiency by providing comprehensive data while minimizing resource usage.
  • This method facilitates more effective target selection and in-depth analysis within screening campaigns.