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A rapid and efficient method for the purification of the complement subcomponents C1r and C1s in zymogen form using
M C Peitsch1, T J Kovacsovics, H Isliker
1Institute of Biochemistry, University of Lausanne, Epalinges, Switzerland.
Journal of Immunological Methods
|April 6, 1988
Abstract:
The purification of the subcomponents C1r and C1s of the first component of complement involves multiple steps and is time-consuming. This accounts for the frequently observed partial activation of the subcomponents. In this report we propose a simplified procedure of purification using a batch method and fast protein chromatography avoiding a shift of pH. The method provides C1r and C1s in a yield of 35 and 60% respectively. In addition, this study provides a simple and sensitive test to assess functional purity of C1r and C1s with respect to the other C1 subcomponents.