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Rapid, sensitive detection of plasma IL-1 by extraction and bioassay
J J Series1, D Shapiro, I Cameron
1Department of Biochemistry, Royal Infirmary, Glasgow, U.K.
Journal of Immunological Methods
|April 6, 1988
Abstract:
A method for separating IL-1 from plasma inhibitors by silica extraction has been developed and coupled to a highly sensitive bioassay using the LBRM TG6 cell line and the I1-2 dependent HT2A cell line. Using this assay we have detected IL-1 activity in plasma from patients undergoing elective surgery.