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Species identification in forensic samples using the SPInDel approach: A GHEP-ISFG inter-laboratory collaborative

Cíntia Alves¹, Rui Pereira¹, Lourdes Prieto²

¹Instituto de Investigação e Inovação em Saúde, I3S, Universidade do Porto, Portugal; Instituto de Patologia e Imunologia Molecular da Universidade do Porto (IPATIMUP), Porto, Portugal.

Forensic Science International. Genetics

|March 22, 2017

Summary

The Species Identification by Insertions/Deletions (SPInDel) method accurately identified species in forensic DNA samples, even with degraded or mixed DNA. This validated method is efficient for casework, demonstrating high success rates in collaborative testing.

Keywords:

Collaborative exercise Forensic investigations SPInDel Species identification mtDNA

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Area of Science:

Forensic science
Molecular biology
Genetics

Background:

DNA analysis is crucial for species identification in forensics.
Validated methods are needed for degraded and low-quality DNA samples.
The Species Identification by Insertions/Deletions (SPInDel) method was recently developed.

Purpose of the Study:

To test and validate the SPInDel method in a collaborative exercise.
To assess the SPInDel method's performance with challenging forensic samples.
To evaluate the SPInDel method's suitability for routine forensic casework.

Main Methods:

A collaborative exercise involving 24 laboratories from 10 countries.
Participants used the SPInDel kit to analyze 11 DNA samples of varying quality and mixtures.
Species identification was based on numeric profiles from six mitochondrial ribosomal RNA (rRNA) gene regions.

Main Results:

22 out of 24 laboratories correctly identified all species.
98.8% of reported SPInDel profiles were accurate, including low DNA quantity and mixture samples.
The SPInDel method demonstrated efficiency in mixture analysis and was easily implemented across different platforms.

Conclusions:

The SPInDel method is a valid and efficient tool for species identification in forensic investigations.
The method performs well with degraded DNA, mixtures, and low DNA amounts.
SPInDel offers a reliable alternative to DNA sequencing for forensic species identification.