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Aptamers for CD Antigens: From Cell Profiling to Activity Modulation.

Amin Nozari1, Maxim V Berezovski1

  • 1Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N6N5, Canada.

Molecular Therapy. Nucleic Acids
|March 23, 2017
PubMed
Summary

Nucleic acid aptamers offer a powerful alternative to antibodies for targeting cell surface proteins (CD proteins). These aptamers have diverse applications in cell biology, diagnostics, and therapies, including cancer and HIV/AIDS treatment.

Keywords:
aptamerscell imagingcluster of differentiationdrug deliverysiRNA

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biomedical Science

Background:

  • Nucleic acid aptamers are emerging as potent alternatives to antibodies due to their specific target binding, cost-effective synthesis, and versatile labeling capabilities.
  • Cluster of differentiation (CD) proteins on cell surfaces are key targets for aptamer development.
  • Anti-CD aptamers hold significant potential in various biomedical fields, including diagnostics and therapeutics for diseases like cancer and HIV/AIDS.

Purpose of the Study:

  • To review and summarize the nucleic acid sequences of anti-CD aptamers.
  • To highlight the validated applications of anti-CD aptamers in scientific research and medicine.

Main Methods:

  • Literature review of validated studies on anti-CD aptamers.
  • Analysis of aptamer sequences and their binding specificities.
  • Compilation of reported applications in cell biology and biomedicine.

Main Results:

  • Aptamers demonstrate strong and specific binding to diverse cell surface CD proteins.
  • Anti-CD aptamers can function as both agonists and antagonists of CD receptors.
  • Aptamers facilitate targeted delivery of therapeutic agents, such as small interfering RNA (siRNA).

Conclusions:

  • Nucleic acid aptamers represent a versatile and promising tool for targeting CD proteins.
  • The reviewed anti-CD aptamers have demonstrated efficacy in various validated applications, paving the way for further development in diagnostics and therapies.
  • The unique properties of aptamers, including their dual agonist/antagonist potential and gene silencing capabilities, underscore their therapeutic and diagnostic value.