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Analysis and isolation of cytomegalovirus DNA by field inversion gel electrophoresis
F M van den Berg1, M Jiwa, R Rook
1Department of Pathology, University of Amsterdam, The Netherlands.
The Journal of General Virology
|March 1, 1988
Abstract:
High molecular weight human cytomegalovirus (CMV) DNA was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. A high yield of CMV DNA molecules was obtained within 1 week of infecting the cell culture. Labelling of the viral DNA with biotin by nick translation enabled the detection of CMV-infected cells in sections of paraffin-embedded human adrenal gland by in situ hybridization.