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Published on: October 7, 2011
Detection of Aleutian disease virus DNA in tissues of naturally infected mink
L Haas1, M Löchelt, O R Kaaden
1Institute of Virology, Hannover Veterinary School, F.R.G.
Abstract:
Organs of naturally infected mink were examined for the presence of Aleutian disease virus (ADV) DNA by in situ hybridization. Spleen, lymph nodes, thymus, bone marrow, kidney, liver, lung and small intestine were found to be positive for ADV to differing extents. Infected lymphoid organs showed a focal distribution of positive cells. Southern blot analysis of DNA extracted from infected organs revealed replicative forms of viral DNA in spleen and bone marrow samples only. These findings are consistent with a lymphotropism of ADV in vivo. Compared to the situation after experimental infection of mink these results indicate additional sites of virus replication and/or persistence of the naturally occurring disease.
Insights
Naturally infected mink show Aleutian disease virus (ADV) DNA in multiple organs, with replication primarily in spleen and bone marrow. This suggests broader viral presence and potential persistence in naturally occurring ADV infections.
Area of Science:
- Veterinary Virology
- Pathology
- Molecular Biology
Background:
- Aleutian disease virus (ADV) causes significant morbidity and mortality in mink.
- Understanding the tissue tropism and replication sites of ADV is crucial for disease management.
Purpose of the Study:
- To investigate the distribution and replication of Aleutian disease virus (ADV) in organs of naturally infected mink.
- To compare viral presence in natural infections versus experimental infections.
Main Methods:
- In situ hybridization was used to detect ADV DNA in various mink organs.
- Southern blot analysis was performed on DNA from infected organs to identify viral DNA forms.
Main Results:
- ADV DNA was detected in spleen, lymph nodes, thymus, bone marrow, kidney, liver, lung, and small intestine.
- Replicative forms of viral DNA were found exclusively in spleen and bone marrow.
- Infected lymphoid organs exhibited a focal distribution of ADV-positive cells.
Conclusions:
- The findings support a lymphotropic pattern for ADV in naturally infected mink.
- Naturally occurring ADV infections may involve additional sites of viral replication or persistence compared to experimental models.
