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Fabrication of Decellularized Cartilage-derived Matrix Scaffolds
Published on: January 7, 2019
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[Development and characterization of oriented scaffolds derived from cartilage extracellular matrix]
Li Kun1, Zhao Yanhong2, Xu Chen2
1Dept. of Orthodontics, Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China;Dept. of Orthodontics, Yantai Stomatological Hospital, Yantai 264000, China.
Summary
Oriented scaffolds from cartilage extracellular matrix (CECM) show promising results for cartilage tissue engineering. These CECM scaffolds exhibit favorable biocompatibility and structural properties for adipose-derived stem cells (ADSCs).
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Cartilage defects pose significant challenges in regenerative medicine.
- Developing biomimetic scaffolds is crucial for effective cartilage repair.
- Cartilage extracellular matrix (CECM) offers a promising natural source for scaffold fabrication.
Purpose of the Study:
- To prepare oriented scaffolds from CECM.
- To characterize the physicochemical properties of these scaffolds.
- To evaluate their compatibility with adipose-derived stem cells (ADSCs).
Main Methods:
- Porcine articular cartilage was processed to obtain nanofibers.
- Decellularization using Triton X-100 yielded CECM.
- Oriented scaffolds were fabricated via unidirectional solidification and lyophilization.
- Scaffolds underwent crosslinking and comprehensive property evaluation.
Main Results:
- Scaffolds exhibited homogeneous porous structures with nanofibers.
- Histological staining confirmed cartilage matrix components.
- High porosity (95.46%) and water absorption (95.89%) were observed.
- A compressive elastic modulus of approximately 40.21 kPa was recorded.
Conclusions:
- CECM-derived scaffolds mimic native cartilage composition and show good biocompatibility.
- The scaffold's porous architecture supports ADSC adhesion, proliferation, and infiltration.
- These oriented CECM scaffolds are optimal for cartilage tissue engineering applications.
