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Lung carcinoma of sheep (jaagsiekte): tissue-bound precipitating antibodies
Firmly bound IgG was examined and its activity was tested in spontaneous lung carcinoma of sheep (jaagsiekte). High levels of IgG were extracted by acid-glycine buffer (GB) at pH 2.8 from both the tumor tissue and normal lung, which were exhaustively prewashed by phosphate-buffered saline (PBS) at pH 7.4. The first acid-GB eluates of the normal or tumor tissue acted in the immunodiffusion test as precipitating antibodies against antigens of the first PBS eluate of the tumor tissue. In addition, free intracytoplasmic type A particles were observed in cell-free tumor extract.
Firmly bound IgG was examined and its activity was tested in spontaneous lung carcinoma of sheep (jaagsiekte). High levels of IgG were extracted by acid-glycine buffer (GB) at pH 2.8 from both the tumor tissue and normal lung, which were exhaustively prewashed by phosphate-buffered saline (PBS) at pH 7.4. The first acid-GB eluates of the normal or tumor tissue acted in the immunodiffusion test as precipitating antibodies against antigens of the first PBS eluate of the tumor tissue. In addition, free intracytoplasmic type A particles were observed in cell-free tumor extract.