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Melaxillines A and B, monoterpenoid indole alkaloids from Melodinus axillaris
Lei Fang1, Shan-Ming Tian2, Jie Zhou1
1College of Biological Sciences and Technology, University of Jinan, Jinan 250022, PR China; Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan 250014, PR China.
Abstract:
Two new monoterpenoid indole alkaloids, melaxillines A (1) and B (2), together with four known alkaloids (3-6), were isolated from the roots of Melodinus axillaris. The structures were determined by extensive spectroscopic methods, including 1D and 2D-NMR spectroscopy, high resolution mass spectrometric data, and by comparison to literature data. New compounds 1-2 might be derived from a common precursor (3) by proposing their biogenetic route, and compound 1 represents the first Melodinus alkaloid featuring an ethyl acetate group skeleton at C-18. The anti-inflammatory effects of all the isolated compounds were evaluated by measuring the inhibitory ratios of β-glucuronidase release in rat polymorphonuclear leukocytes (PMNs) induced by platelet-activating factor. Compounds 1-4 showed significant anti-inflammatory activities with IC50 values ranging from 1.51 to 21.56μM, respectively.
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