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Dentin bond optimization using the dimethyl sulfoxide-wet bonding strategy: A 2-year in vitro study
Thiago Henrique Scarabello Stape1, Leo Tjäderhane2, Arzu Tezvergil-Mutluay3
1Adhesive Dentistry Research Group, Institute of Dentistry, University of Turku, Lemminkaisenkatu 2, 20520 Turku, Finland; Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas, Av. Limeira Piracicaba, SP 13414-903, Brazil.
Dimethyl sulfoxide (DMSO)-wet bonding improved long-term dental adhesive strength and durability. This biomodification technique enhances hybrid layer integrity, reducing degradation for synthetic biomaterials bonded to dentin.
Area of Science:
- Dental biomaterials science
- Adhesive dentistry
- Polymer science
Background:
- Dentin bonding is challenged by its inherent hydration.
- Synthetic polymeric biomaterials require robust interfacial interactions with dentin.
- Long-term durability of adhesive bonds is crucial for clinical success.
Purpose of the Study:
- To evaluate the efficacy of dimethyl sulfoxide (DMSO)-wet bonding for enhancing long-term dental adhesive performance.
- To assess the impact of DMSO pretreatment on the hybrid layer and bond strength over time.
- To investigate the influence of DMSO on the degree of conversion and nanoleakage in different adhesive systems.
Main Methods:
- Sound human third molars were restored using total-etch (SBMP) and self-etch (CF) adhesives, with and without DMSO pretreatment.
- Micro-Raman spectroscopy, micro-tensile bond strength testing, fracture analysis, and nanoleakage evaluation were performed at 24 hours, 1 year, and 2 years.
- Sample groups included n=10 teeth per condition for each analysis.
Main Results:
- DMSO-wet bonding significantly increased 24-hour bond strengths for SBMP, sustained over two years with fewer adhesive failures.
- DMSO-treated CF samples showed significantly higher bond strength at two years compared to untreated samples.
- DMSO reduced adhesive failures at two years for both adhesive systems and decreased nanoleakage in SBMP samples after aging.
Conclusions:
- Dimethyl sulfoxide (DMSO) biomodification of dentin creates more durable hybrid layers resistant to hydrolytic degradation.
- DMSO-wet bonding is effective for both self-etch and etch-and-rinse bonding mechanisms, improving long-term bond stability.
- The application of DMSO enhances the longevity of the adhesive interface between synthetic biomaterials and dentin.
