Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

90.4K
Overview
90.4K
The Extracellular Matrix01:29

The Extracellular Matrix

12.9K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
12.9K
Extracellular Matrix01:26

Extracellular Matrix

6.2K
Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SNED1 modulates ECM architecture and cell proliferation via its LDV integrin-binding motif.

Journal of cell science·2026
Same author

Zika virus infections of human stem cell-derived cerebral organoids reveal viral lineage-specific pathogenesis responses.

mBio·2026
Same author

Multisite Assessment of Methods for Cell Preservation Upstream of Single-Cell RNA Sequencing.

Journal of biomolecular techniques : JBT·2026
Same author

Identification of Pancreatic Ductal Adenocarcinoma Extracellular Matrix Signatures from In-Depth Proteomic Profiling that Correlate with Lymphocyte Infiltration.

Cancer research communications·2026
Same author

Deletion of low-essentiality, secretion-associated genes enhances recombinant protein production in Komagataella phaffii.

Microbial cell factories·2026
Same author

MatriSpace: Identification and visualization of spatially resolved ECM gene expression patterns in health and disease.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Mar 5, 2026

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

17.5K

Comprehensive proteomic characterization of stem cell-derived extracellular matrices.

Héloïse Ragelle1, Alexandra Naba1, Benjamin L Larson2

  • 1David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02142, USA.

Biomaterials
|March 23, 2017
PubMed
Summary

Cell-derived extracellular matrices (ECM) better mimic the stem cell niche than standard cultures. Characterizing ECM protein composition reveals unique signatures that influence stem cell behavior, guiding biomaterial design for tissue engineering.

Keywords:
ECM cell-culture substrateExtracellular matrixMesenchymal stem cellProteomic analysisStem-cell derived matricesTissue engineering

More Related Videos

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
07:28

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Published on: July 23, 2015

28.6K
Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
14:02

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

13.2K

Related Experiment Videos

Last Updated: Mar 5, 2026

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

17.5K
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
07:28

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Published on: July 23, 2015

28.6K
Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry
14:02

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

Published on: April 21, 2017

13.2K

Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Proteomics

Background:

  • The stem cell niche's extracellular matrix (ECM) regulates stem cell function through cell-ECM interactions.
  • Cell-derived ECMs offer in vitro models of the native stem cell microenvironment.
  • Mesenchymal stem cells (MSCs) show altered behavior on ECM substrates compared to standard tissue-culture polystyrene (TCPS).

Purpose of the Study:

  • To characterize and compare the proteomic composition of ECMs derived from different cell types (bone marrow MSC, adipose MSC, neonatal fibroblasts).
  • To understand how these cell-derived ECMs influence stem cell behavior and gene expression.
  • To inform the design of biomaterials for stem cell culture and tissue engineering.

Main Methods:

  • Quantitative proteomic analysis to determine the protein composition of cell-derived ECMs.
  • In vitro cell culture experiments comparing cell responses on different ECM substrates and TCPS.
  • Evaluation of cell survival and gene expression profiles.

Main Results:

  • Each cell-derived ECM exhibited a unique 'matrisome' signature but shared common proteins.
  • Cells cultured on ECM substrates showed differential survival and gene expression compared to TCPS.
  • The impact of ECM substrates varied depending on the cell type, indicating cell-specific responses.

Conclusions:

  • Cell-derived ECMs provide distinct microenvironments that modulate stem cell behavior.
  • Understanding ECM composition is crucial for developing effective stem cell culture systems and biomaterials.
  • This approach aids in designing defined systems that mimic native ECM signals for regenerative medicine.