Hybrid Core-Shell (HyCoS) Nanoparticles produced by Complex Coacervation for Multimodal Applications

Area of Science:

• Biomaterials Science
• Nanotechnology
• Medical Imaging

Background:

• Multimodal imaging probes combine diagnostic information from different modalities.
• Nanoparticles (NPs) can encapsulate multiple imaging tracers for simultaneous diagnostic techniques.
• Existing methods for nanoparticle synthesis may lack stability or efficiency.

Purpose of the Study:

• To develop novel, biocompatible core-shell polymeric nanoparticles (HyCoS) for multimodal imaging applications.
• To improve the stability and performance of nanoparticles for simultaneous MRI and optical imaging.
• To explore the potential of HyCoS for triggered drug release.

Main Methods:

• Utilized a complex coacervation process with controlled reaction temperature to synthesize HyCoS nanoparticles.
• Developed a double-crosslinked system to enhance nanostructure stability with Gd-DTPA (MRI contrast agent).
• Loaded HyCoS with dyes (ATTO 633) or conjugated with FITC for optical imaging capabilities.

Main Results:

• Achieved a six-fold increase in relaxometric properties of Gd-DTPA through controlled crosslinking.
• Demonstrated stable core-shell nanoparticles suitable for both MRI and optical imaging.
• Showcased radiation-free detection capabilities for in vivo applications.
• Reported preliminary data on pH-triggered drug release.

Conclusions:

• HyCoS nanoparticles offer a stable and versatile platform for multimodal imaging.
• The developed nanoparticles enhance MRI contrast and enable sensitive optical imaging.
• HyCoS holds promise for advanced, radiation-free diagnostic tools and targeted drug delivery.