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Microbiota and reproducibility of rodent models
Craig L Franklin1, Aaron C Ericsson1
1Department of Veterinary Pathobiology at the University of Missouri in Columbia, Missouri.
Lab Animal
|March 23, 2017
Summary
Gut microbiota (GM) variability in animal models impacts research reproducibility. This review identifies common GM disruption sources in rodents and suggests strategies to improve experimental consistency.
Area of Science:
- Microbiology
- Animal Models
- Research Reproducibility
Background:
- The gut microbiota (GM) is integral to host health and disease.
- GM disruptions significantly influence animal model phenotypes.
- Variability in GM introduces complexity into preclinical research.
Purpose of the Study:
- To review common sources of gut microbiota variability in rodent models.
- To discuss methods for mitigating GM variability.
- To enhance the reproducibility of animal research.
Main Methods:
- Literature review of studies on gut microbiota in rodent models.
- Analysis of factors contributing to GM variability.
- Synthesis of strategies for controlling GM.
Main Results:
- Identified key sources of GM variability including diet, housing, and experimental procedures.
- Highlighted the impact of these variables on animal phenotypes.
- Outlined practical approaches to standardize GM.
Conclusions:
- Standardizing GM in rodent models is crucial for reliable research.
- Addressing GM variability improves the translatability of findings.
- Implementing consistent protocols enhances experimental reproducibility.
