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Related Experiment Videos

Genetically transformed maize plants from protoplasts.

C A Rhodes1, D A Pierce, I J Mettler

  • 1Sandoz Crop Protection Corporation, Palo Alto, CA 94304-1104.

Science (New York, N.Y.)
|April 8, 1988
PubMed
Summary

Genetically engineered maize plants were successfully created using recombinant DNA technology. This involved transforming maize protoplasts with a neomycin phosphotransferase (NPT II) gene, leading to kanamycin-resistant plants confirmed by Southern analysis.

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Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Genetics

Background:

  • Maize (Zea mays) is a vital global crop requiring advanced genetic improvement.
  • Efficient methods for genetic transformation are crucial for crop development.

Purpose of the Study:

  • To develop a method for genetically transforming maize using protoplast technology.
  • To confirm the integration and expression of a foreign gene in maize.

Main Methods:

  • Maize protoplasts were isolated from embryogenic cell suspension cultures.
  • Electroporation was used to introduce plasmid DNA containing the neomycin phosphotransferase (NPT II) gene.
  • Transformed cells were selected using kanamycin and regenerated into whole plants.

Main Results:

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  • Selected cell lines exhibited NPT II activity, indicating successful gene expression.
  • Southern blot analysis confirmed the presence of the NPT II gene in regenerated maize plants.
  • Transformed plants were grown to maturity.

Conclusions:

  • Electroporation of maize protoplasts is an effective method for genetic transformation.
  • The NPT II gene was stably integrated and functional in the regenerated maize plants.
  • This technique provides a foundation for further genetic engineering of maize.