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Extraction and bioactive profile of the compounds produced by Rhodococcus sp. VLD-10.

Bokka Yellamanda1, Muvva Vijayalakshmi2, Alapati Kavitha3

  • 1Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur, 522 510, India.

3 Biotech
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PubMed
Summary
This summary is machine-generated.

Marine actinobacteria Rhodococcus sp. VLD-10 yielded five bioactive compounds. The compound 3-isopropylhexahydro-1H-pyrido[1,2-a] pyrazine-1,4(6H)-dione showed significant antibacterial activity against various pathogens.

Keywords:
3-Isopropylhexahydro-1H-pyrido[1,2-a] pyrazine-1,4(6H)-dioneActinobacteriaBioactive compoundsRhodococcus

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Area of Science:

  • Microbiology
  • Natural Product Chemistry
  • Pharmacology

Background:

  • Marine actinobacteria are a rich source of novel bioactive compounds.
  • Rhodococcus species are known for producing diverse secondary metabolites with antimicrobial properties.

Purpose of the Study:

  • To isolate and identify bioactive compounds from marine actinobacteria.
  • To elucidate the antimicrobial activity of the isolated compounds.

Main Methods:

  • Isolation and identification of actinobacterial strain using conventional and 16S rRNA sequencing.
  • Cultivation, extraction, purification, and structural elucidation of compounds using chromatographic and spectroscopic techniques.
  • Antimicrobial activity testing (minimum inhibition concentration) against various bacterial and fungal strains.

Main Results:

  • Rhodococcus sp. VLD-10 was isolated from marine samples.
  • Five compounds were isolated and identified: benzoic acid, 2-nitrobenzaldehyde, 4-chlorobenzaldehyde, nonadecanoic acid, and 3-isopropylhexahydro-1H-pyrido[1,2-a] pyrazine-1,4(6H)-dione.
  • 3-isopropylhexahydro-1H-pyrido[1,2-a] pyrazine-1,4(6H)-dione exhibited potent antibacterial activity against Gram-positive and Gram-negative bacteria, with less activity against fungi.

Conclusions:

  • Rhodococcus sp. VLD-10 is a promising source of antimicrobial compounds.
  • The natural occurrence and bioactivity of 3-isopropylhexahydro-1H-pyrido[1,2-a] pyrazine-1,4(6H)-dione from this strain are reported for the first time.
  • Further investigation into these compounds could lead to the development of new antimicrobial agents.