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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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Orchestrating differential data access for translational research: a pilot implementation.

Marco Brandizi1, Olga Melnichuk2, Raffael Bild3

  • 1European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, CB10 1SD, UK. brandizi@ebi.ac.uk.

BMC Medical Informatics and Decision Making
|March 24, 2017
PubMed
Summary
This summary is machine-generated.

Researchers can use common open source software to build secure systems for accessing sensitive biomedical data. This pilot system demonstrates a feasible approach for managing differential access to research data, offering valuable lessons for similar projects.

Keywords:
Biomedical DataClinical DataData AccessHealth Data ProtectionTranslational Research

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Area of Science:

  • Biomedical Informatics
  • Data Security
  • Biobanking Research

Background:

  • Translational researchers require IT solutions for diverse data types, including sensitive patient information.
  • Managing access to human data involves complex ethical, social, and legal considerations like confidentiality and consent.
  • Differential access management is crucial for sensitive data in research settings.

Purpose of the Study:

  • To present a pilot system for coordinating access to heterogeneous biomedical data repositories.
  • To demonstrate the use of common open source software for managing open and restricted access data.
  • To address the need for secure and differential access to sensitive biobanking and biosample research data.

Main Methods:

  • Assembled and configured common open source software components.
  • Implemented a digital identity federation for access control.
  • Developed software to manage resource access entitlements for heterogeneous data repositories.

Main Results:

  • The pilot system successfully allows for differential restricted access based on data protection needs.
  • Performance, feasibility, and reproducibility of the pilot infrastructure were assessed.
  • Common open source software proved sufficient for creating a secure differential access system.

Conclusions:

  • Common open source software can create secure systems for differential access to sensitive data.
  • The pilot implementation offers practical experience and lessons for researchers with similar data access requirements.
  • The system architecture can be extended for more complex data access scenarios.