Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cellular vitamin A-binding proteins in the testis.

D E Ong1, S Takase, F Chytil

  • 1Departments of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.

Annals of the New York Academy of Sciences
|January 1, 1987
PubMed
Summary

Vitamin A is crucial for sperm production (spermatogenesis) in the testis. Specific binding proteins help retinol and retinoic acid regulate gene expression, supporting testicular function and germ cell development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inflammatory bowel disease cancer surveillance in a tertiary referral hospital: attitudes and practice.

Internal medicine journal·2013
Same author

Tissue differences in antigenic properties of non-histone protein-DNA complexes.

Nature: New biology·2010
Same author

Retinoic acid biosynthesis by normal human breast epithelium is via aldehyde dehydrogenase 6, absent in MCF-7 cells.

Cancer research·2001
Same author

Gene duplication gives rise to a new 17-kilodalton lipocalin that shows epididymal region-specific expression and testicular factor(s) regulation.

Endocrinology·2001
Same author

Cellular retinoic acid binding protein is associated with mitochondria.

FEBS letters·2001
Same author

Plasma retinol binding protein: structure and function of the prototypic lipocalin.

Biochimica et biophysica acta·2000

Area of Science:

  • Reproductive biology
  • Endocrinology
  • Molecular biology

Background:

  • Vitamin A is essential for male reproductive health and spermatogenesis.
  • Cellular retinol-binding proteins (CRBP) and cellular retinoic acid-binding proteins (CRABP) are involved in vitamin A metabolism within the testis.

Purpose of the Study:

  • To investigate the specific roles of retinol and retinoic acid in testicular function.
  • To explore the involvement of CRBP and CRABP in vitamin A's action on spermatogenesis and gene expression.

Main Methods:

  • Analysis of cellular localization of CRBP and CRABP in testicular cells.
  • Demonstration of distinct binding sites for retinol and retinoic acid in testicular nuclei and chromatin.

Main Results:

Related Experiment Videos

  • Cellular localization suggests retinoic acid is involved in later germ cell differentiation, while retinol is initially taken up by Sertoli cells.
  • Specific binding sites for retinol and retinoic acid are found in testicular chromatin, requiring their respective binding proteins for revelation.

Conclusions:

  • Vitamin A, via retinol and retinoic acid, regulates gene expression in the testis, likely through direct interaction with chromatin.
  • CRBP and CRABP are crucial for the action of vitamin A metabolites in testicular cells, influencing spermatogenesis and germ cell differentiation.