Related Experiment Video
Updated: Mar 5, 2026
Ultra-High-Speed Western Blot using Immunoreaction Enhancing Technology
Published on: September 26, 2020
Immunological diagnosis of human hydatid cyst using Western immunoblotting technique
Mahboubeh Hadipour1, Mohammad Nazari2, Behnam Sanei2
1Department of Medical Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran.
Background:
Echinococcosis is a parasitic disease with worldwide distribution which is caused by the tapeworms Echinococcus granulosus. Diagnosis of the disease relies on imaging techniques, but the techniques are not able to differentiate the cyst from benign or malignant tumors; hence, appropriate serologic methods are required for the differential diagnosis of the infection.
Materials And Methods:
In this investigation, different sheep hydatid cyst antigens probed with thirty sera of patients with hydatid cyst and also thirty human normal sera using Western immunoblotting technique. Considering results of surgery as gold standard, sensitivity and specificity of Western blotting was estimated.
Results:
Sera of 29, 26, and 16 patients with hydatid cyst reacted with specific bands of hydatid cyst fluid (HCF), protoscolex crude antigen, and cyst wall crude antigen, respectively. However, none of the normal human sera reacted with those specific bands.
Conclusion:
A 20 kDa band of sheep HCF is an appropriate antigen for serodiagnosis of hydatid cyst infection.
Related Concept Videos
01:15Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
01:33Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...