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Related Concept Videos

Western Blotting01:15

Western Blotting

21.4K
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
21.4K

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Related Experiment Video

Updated: Mar 5, 2026

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
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Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

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Profiling protein expression in circulating tumour cells using microfluidic western blotting.

Elly Sinkala1, Elodie Sollier-Christen2,3, Corinne Renier2,3

  • 1Department of Bioengineering, University of California, Berkeley, 308B Stanley Hall, MC 1762, Berkeley, California 94720, USA.

Nature Communications
|March 24, 2017
PubMed
Summary

This study introduces a microfluidic western blot to analyze protein expression in individual circulating tumor cells (CTCs) from breast cancer patients, revealing unique cellular properties and protein subpopulations.

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Area of Science:

  • Oncology
  • Biotechnology
  • Proteomics

Background:

  • Circulating tumor cells (CTCs) are rare cells in cancer patients' blood, with their clinical significance under investigation.
  • Characterizing CTCs through protein profiling can complement genomic and transcriptomic analyses, aiding in understanding their biology.

Purpose of the Study:

  • To develop and validate a microfluidic western blot for multiplexed protein analysis of individual CTCs.
  • To investigate the biophysical properties and protein expression patterns of CTCs from estrogen receptor-positive (ER+) breast cancer patients.

Main Methods:

  • A microfluidic western blot assay was designed for an eight-plex protein panel.
  • The assay was applied to individual CTCs isolated from ER+ breast cancer patients.
  • Analysis focused on protein expression, lysis resistance, and cell-specific quantification.

Main Results:

  • The assay successfully analyzed protein expression in scarce patient-derived CTCs.
  • Patient-derived CTCs exhibited a lysis-resistant biophysical phenotype compared to cell lines.
  • Two distinct subpopulations of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were identified within CTCs.

Conclusions:

  • Single-CTC proteomics using this microfluidic platform provides a novel method for CTC characterization.
  • Archivable, multiplexed protein analysis offers a complementary approach to understanding CTC biology and clinical impact.