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Tagging and Fusion Proteins

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Related Experiment Video

Updated: Mar 5, 2026

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

PeptideMapper: efficient and versatile amino acid sequence and tag mapping.

Dominik Kopczynski¹, Harald Barsnes^2,3, Pål R Njølstad^4,5

¹Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V, Dortmund 44227, Germany.

Bioinformatics (Oxford, England)

|March 24, 2017

Summary

This summary is machine-generated.

PeptideMapper, a novel software tool, aids in peptide identification and analysis within proteomics research. Its open-source nature promotes accessibility and collaborative development in the field.

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Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Area of Science:

Proteomics
Bioinformatics
Computational Biology

Background:

Accurate peptide identification is crucial for protein research.
Existing tools may have limitations in flexibility or accessibility.

Purpose of the Study:

To introduce PeptideMapper, a new software tool for peptide analysis.
To provide an open-source and accessible solution for the scientific community.

Main Methods:

PeptideMapper is developed using the Java programming language.
The software is part of the open-source CompOmics framework.
It is released under the Apache 2.0 license.

Main Results:

PeptideMapper offers a flexible platform for peptide mapping.
The open-source implementation facilitates integration and further development.

Conclusions:

PeptideMapper enhances capabilities in peptide identification and analysis.
Its open-source availability promotes wider adoption and research advancement.