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Cell Factory Engineering.

Anne Mathilde Davy¹, Helene Faustrup Kildegaard², Mikael Rørdam Andersen¹

¹Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.

|March 24, 2017

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This meta-review consolidates 33 generic strategies for cell factory engineering, applicable across diverse cell types and products. It offers guides for rational engineering to produce metabolites, pathways, and proteins.

Area of Science:

Biotechnology and Synthetic Biology
Metabolic Engineering

Background:

Cell factory engineering is crucial for producing molecules of interest, encompassing native metabolite production, heterologous pathway expression, and protein expression.
Existing reviews often focus on specific strategies or cell types, lacking a unified overview.

Purpose of the Study:

To provide a comprehensive meta-review of cell factory engineering by consolidating existing knowledge.
To identify and categorize generic strategies applicable across various cell types and production targets.

Main Methods:

Conducted a meta-analysis of over 130 reviews and key studies in cell factory engineering.
Identified and classified 33 generic engineering strategies.

Main Results:

Keywords:

Aspergillus Bacillus subtilis CHO cells Escherichia coli Pichia Saccharomyces cerevisiae cell factory cell factory engineering metabolic engineering protein secretion

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Identified 33 generic strategies applicable to multiple cell types and products.
Categorized strategies into three main areas: native metabolite production, heterologous pathway expression, and protein expression.
Demonstrated success of these strategies across major cell types.

Conclusions:

Cell factory engineering offers a unified approach to rational cell modification for diverse molecular production.
The identified generic strategies provide a foundational guide for broad applications in cell factory engineering.
This meta-review serves as a valuable resource for researchers in synthetic biology and biotechnology.