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Draft Genome Sequence of Providencia stuartii PS71, a Multidrug-Resistant Strain Associated with Nosocomial

A Liakopoulos1, O Oikonomou2, D W Wareham3,4

  • 1Department of Bacteriology and Epidemiology, CVI of Wageningen University, Lelystad, The Netherlands.

Genome Announcements
|March 25, 2017
PubMed

Insights

This study details the genome of a pan-resistant Providencia stuartii strain. This multidrug-resistant bacterium was involved in a Greek hospital outbreak, highlighting significant antimicrobial resistance challenges.

Area of Science:

  • Microbiology and Infectious Diseases
  • Genomics and Molecular Biology
  • Antimicrobial Resistance

Background:

  • Providencia stuartii is a significant pathogen in healthcare settings, often linked to hospital-acquired infections.
  • This bacterium exhibits intrinsic resistance to numerous antimicrobial agents, complicating treatment strategies.
  • The emergence of acquired resistance genes further exacerbates the challenge posed by P. stuartii.

Purpose of the Study:

  • To perform a draft genome sequencing of a P. stuartii strain implicated in a hospital outbreak.
  • To identify specific acquired resistance genes contributing to the strain's panresistance phenotype.
  • To understand the genetic basis of multidrug resistance in this outbreak-associated strain.

Main Methods:

  • Whole-genome sequencing was employed to determine the complete genetic makeup of the P. stuartii isolate.
  • Bioinformatic analysis was used to identify known antimicrobial resistance genes within the sequenced genome.
  • Comparative genomics may be used to understand the evolutionary context of resistance gene acquisition.

Main Results:

  • The draft genome sequence revealed the presence of acquired resistance genes conferring resistance to cephalosporins (blaSHV-5, blaVEB-1), carbapenems (blaVIM-1), and aminoglycosides (rmtB).
  • These genetic determinants explain the panresistance observed in the P. stuartii strain.
  • The strain was identified as being involved in a nosocomial outbreak within Greek hospitals.

Conclusions:

  • The genetic characterization of this P. stuartii strain provides crucial insights into the mechanisms of panresistance.
  • The findings underscore the threat of multidrug-resistant bacteria in hospital environments and the need for effective surveillance.
  • Understanding the genomic basis of resistance is vital for developing targeted interventions against P. stuartii outbreaks.

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