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SERS-Based Flavonoid Detection Using Ethylenediamine-β-Cyclodextrin as a Capturing Ligand.

Jae Min Choi1, Eunil Hahm2, Kyeonghui Park3

  • 1Center for Biotechnology Research in UBITA (CBRU), Institute for Ubiquitous Information Technology and Application (UBITA), Konkuk University, Seoul 05029, Korea. jm.choi.25@gmail.com.

Nanomaterials (Basel, Switzerland)
|March 25, 2017
PubMed
Summary

This study presents a novel sensor using ethylenediamine-modified β-cyclodextrin on silver nanoparticles embedded in silica. This sensor effectively detects flavonoids with high sensitivity and selectivity.

Keywords:
cyclodextrinethylenediamine cyclodextrinflavonoidssurface-enhanced Raman scattering (SERS)

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Area of Science:

  • Nanomaterials Science
  • Analytical Chemistry
  • Biochemistry

Background:

  • Flavonoids are important plant compounds with various health benefits.
  • Sensitive and selective detection methods for flavonoids are crucial for research and diagnostics.
  • Surface-enhanced Raman scattering (SERS) offers high sensitivity for molecular detection.

Purpose of the Study:

  • To develop a novel SERS substrate for effective flavonoid detection.
  • To immobilize ethylenediamine-modified β-cyclodextrin (Et-β-CD) on silver nanoparticle-embedded silica nanoparticles (SiO₂@Ag@Et-β-CD NPs).
  • To evaluate the sensor's performance in detecting various flavonoids.

Main Methods:

  • Synthesis of aggregated silver nanoparticle-embedded silica nanoparticles (SiO₂@Ag NPs).
  • Immobilization of ethylenediamine-modified β-cyclodextrin (Et-β-CD) onto SiO₂@Ag NPs.
  • Utilizing the resulting SiO₂@Ag@Et-β-CD NPs as a SERS substrate for flavonoid detection.
  • Characterization using ultraviolet absorption spectra and SERS measurements.

Main Results:

  • The SiO₂@Ag@Et-β-CD NPs demonstrated enhanced ultraviolet absorption upon flavonoid complexation.
  • The sensor exhibited high sensitivity for detecting flavonoids like luteolin in the concentration range of 10⁻⁷ to 10⁻³ M.
  • The SERS signal for luteolin was significantly stronger compared to other organic molecules.
  • The sensor showed excellent stability, with the SERS signal for luteolin persisting after repeated washing.

Conclusions:

  • SiO₂@Ag@Et-β-CD NPs serve as an effective SERS substrate for flavonoid detection.
  • The developed sensor is rapid, sensitive, and selective for identifying flavonoids.
  • This nanomaterial-based sensor holds promise for future applications in flavonoid analysis.