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Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Published on: March 15, 2019
From rabbit antibody repertoires to rabbit monoclonal antibodies
Justus Weber1, Haiyong Peng1, Christoph Rader1
1Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, USA.
Abstract:
In this review, we explain why and how rabbit monoclonal antibodies have become outstanding reagents for laboratory research and increasingly for diagnostic and therapeutic applications. Starting with the unique ontogeny of rabbit B cells that affords highly distinctive antibody repertoires rich in in vivo pruned binders of high diversity, affinity and specificity, we describe the generation of rabbit monoclonal antibodies by hybridoma technology, phage display and alternative methods, along with an account of successful humanization strategies.
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