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Isolation and characterization of microsatellite loci for Smilax sieboldii (Smilacaceae)
Yalu Ru1, Ruijing Cheng2, Jing Shang2
1Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China; Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Ecology and Conservation Center for Gene Resources of Endangered Wildlife, Zhejiang University, Hangzhou 310058, People's Republic of China.
Premise Of The Study:
Polymorphic microsatellite markers were developed for Smilax sieboldii (Smilacaceae), a member of the S. hispida group with a biogeographic disjunction between eastern Asia and North America, to study the phylogeography and incipient speciation of this species and its close relatives.
Methods And Results:
Transcriptome sequencing produced 47,628 unigenes. Seventeen loci were developed from 122 randomly selected primer pairs. Polymorphism and genetic variation were evaluated for 68 accessions representing five populations of S. sieboldii. The number of alleles per locus ranged from four to 18; the expected heterozygosity ranged from 0.59 to 0.92. Twelve loci were successfully amplified in five related species: S. scobinicaulis, S. californica, S. hispida, S. moranensis, and S. jalapensis.
Conclusions:
These novel expressed sequence tag-derived microsatellite markers will facilitate further population genetic research of S. sieboldii and its close allies of the S. hispida group.
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