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Updated: Mar 5, 2026

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Published on: February 19, 2020
Development and Application of a Brush-Spray Derived from a Calligraphy-Brush-Style Synthetic Hair Pen for Use in
Jen-Ying Liu1, Pei-Chun Chen1, Yea-Wenn Liou1
1Department of Chemistry, National Taiwan Normal University.
Abstract:
The development of a novel type of a sampling/ionization kit for use in electrospray ionization/mass spectrometry is reported. Using a small calligraphy-brush-style synthetic hair pen (nylon-brush), and analogous to paper-spray mass spectrometry, the analytes can be collected, elution/desorption and then ionized from the surface of the nylon-brush. The body of the kit was produced by means of a commercial 3D-printer, in which ABS (acrylonitrile butadiene styrene) was used as the starting material. Meanwhile, a small nylon-brush was embedded inside a 3D-printed plastic cell, in which a solvent was supplied to rinse the brush by means of capillary action. The size and weight of the kit were 1 g and 4 cm, respectively. The kit is disposable and it has various functions, including non-invasive sampling, sample-evaporation and ionization. As a result, when a type of pesticide was selected as the test sample (dimethoate; C5H12NO3PS2), the limit of detection was determined to be 0.1 μg/mL. Collecting the pesticide from a leaf-surface (lettuce) was also successful. The process for fabricating the nylon-brush kit and the optimized experimental conditions are reported herein.
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