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Updated: Mar 5, 2026

Author Spotlight: MAPP Protocol – Advancing Glycan Analysis
Published on: September 29, 2023
A Rapid Approach for Fabricating Boronic Acid-Functionalized Plates for On-Probe Detection of Glycoprotein and
Yu-Ching Liu1, Chao-Jung Chen2
1Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital.
Abstract:
We developed a rapid and simple approach without using complex mechanical or chemical protocols to fabricate boronic acid-functionalized plates for glycoprotein or glycopeptide enrichment and mass spectrometry (MS) analysis. By coating the boronic acid-functionalized silica particles on a polydimethylsiloxane (PDMS)-coated matrix-assisted laser desorption/ionization (MALDI) plate, these particles can form a firmly monolayer of particles on PDMS membrane for sample handling without peeling off. The boronic acid particles-coated PDMS plate (BP plate) was successfully applied to the enrichment of horseradish peroxidase (HRP) protein and their digested glycopeptides.

