Comparisons between two receptor assays for 1,25-dihydroxyvitamin D

We present a competitive protein binding assay (CPBA) for 1,25(OH)2D employing 1,25(OH)2D receptor from calf thymus, which was compared with a CPBA-employing receptor from rachitic chick intestine. The thymus receptor assay was more sensitive, specific and precise than the intestinal receptor assay. The thymus receptor assay measured both 1,25(OH)2D2 and 1,25(OH)2D3 with equal affinity, whereas 1,25(OH)2D2 was 1.1 times less potent than 1,25(OH)2D3 in the displacement from the chick intestinal receptor. Mean serum values of 1,25(OH)2D in normal subjects, post-menopausal women, pregnant women, and patients with chronic renal failure measured by the two assay systems did not differ. Furthermore, both assays showed that 1,25(OH)2D was unchanged in post-menopausal women after treatment with vitamin D2 or vitamin D3, 4000 IU/day for 8 weeks. We conclude that the high sensitivity of the thymus receptor and the equal affinity for the D2 and D3 analogue make the thymus receptor assay a reliable alternative to the chick intestinal receptor assay.