Biochemical model of C₃ photosynthesis applied to wheat at different temperatures

We examined the effects of leaf temperature on the estimation of maximal Rubisco capacity (V_cmax ) from gas exchange measurements of wheat leaves using a C₃ photosynthesis model. Cultivars of spring wheat (Triticum aestivum (L)) and triticale (X Triticosecale Wittmack) were grown in a greenhouse or in the field and measured at a range of temperatures under controlled conditions in a growth cabinet (2 and 21% O₂ ) or in the field using natural diurnal variation in temperature, respectively. Published Rubisco kinetic constants for tobacco did not describe the observed CO₂ response curves well as temperature varied. By assuming values for the Rubisco Michaelis-Menten constants for CO₂ (K_c ) and O₂ (K_o ) at 25 °C derived from tobacco and the activation energies of V_cmax from wheat and respiration in the light, R_d , from tobacco, we derived activation energies for K_c and K_o (93.7 and 33.6 kJ mol^-1 , respectively) that considerably improved the fit of the model to observed data. We confirmed that temperature dependence of dark respiration for wheat was well described by the activation energy for R_d from tobacco. The new parameters improved the estimation of V_cmax under field conditions, where temperatures increased through the day.