Related Experiment Video
Updated: Mar 5, 2026
Studying Orthodontic Tooth Movement in Mice
Published on: August 2, 2024
Does systemic administration of casein phosphopeptides affect orthodontic movement and root resorption in rats?
Lachlan Crowther1, Gang Shen2, Mohammed Almuzian1,3
1Discipline of Orthodontics, Faculty of Dentistry, University of Sydney, New South Wales, Australia.
Objectives:
To assess the potential effects of casein phosphopeptides (CPPs) on orthodontically induced iatrogenic root resorption (OIIRR) and orthodontic teeth movement.
Materials And Methods:
Forty Wistar rats (aged 11 weeks) were randomly divided into experimental group (EG; n = 20) that received a diet supplemented with CPP and control group (CG; n = 20) devoid of diet supplement. A 150 g force was applied using nickel titanium (NiTi) coil that was bonded on maxillary incisors and extended unilaterally to a maxillary first molar. At Day 28, animals in both groups were euthanized. Volumetric assessment of root resorption craters and linear measurement of maxillary first molars movement were blindly examined using a micro-computed tomography scan.
Results:
Nine rats were excluded from the experiment due to loss during general anesthesia or appliances' failure. Intra-operator reproducibility was high in both volumetric and linear measurements, 92.8 per cent and 98.5-97.6 per cent, respectively. The results reveal that dietary CPP has statistically insignificant effect on the overall OIIRR and orthodontic movement.
Conclusions:
CPP seems to have statistically insignificant effect on the volume of OIIRR and orthodontic movement in rats. A long-term study with larger sample size using a different concentration of CPP is required to clarify the dentoalveolar effect of CPP.
