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FunResDB-A web resource for genotypic susceptibility testing of Aspergillus fumigatus
Michael Weber1, Jonas Schaer1, Grit Walther1
1German National Reference Center for Invasive Fungal Infections - NRZMyk, Septomics Research Centre, Leibniz-Institute for Natural Product Research and Infection Biology - Hans-Knoell-Institute, 07745 Jena, Germany.
Abstract:
Therapy of invasive aspergillosis is becoming more difficult due to the emergence of azole resistance in Aspergillus fumigatus. A majority of resistant strains carries mutations in the CYP51A gene. Due to a lack of sensitivity of culture-based methods, molecular detection of A. fumigatus has become an important diagnostic tool. We set up the database FunResDB (www.nrz-myk.de/funresdb) to gather all available information about CYP51A-dependent azole resistance from published literature. In summary, the screening resulted in 79 CYP51A variants, which are linked to 59 nonsynonymous mutations. A tailor-made online sequence analysis tool allows for genotypic susceptibility testing of A. fumigatus.
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