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SNP detection using RNA-sequences of candidate genes associated with puberty in cattle
M M Dias1, A Cánovas2, C Mantilla-Rojas3
1Departamento de Zootecnia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, SP, Brasil marina.mortati@gmail.com.
Genetics and Molecular Research : GMR
|March 25, 2017
Summary
Researchers identified 1157 single nucleotide polymorphisms (SNPs) in 62 cattle genes linked to heifer puberty. Of these, 172 SNPs were consistent across five breeds, aiding genetic selection for improved fertility.
Area of Science:
- Animal Genetics
- Reproductive Biology
- Genomics
Background:
- Heifer pregnancy is a key economic trait in cattle production.
- Genetic selection programs aim to improve fertility traits.
- Understanding the genetic basis of heifer puberty is crucial for breeding programs.
Purpose of the Study:
- To identify single nucleotide polymorphisms (SNPs) associated with heifer puberty.
- To analyze RNA-sequencing (RNA-Seq) data from various cattle tissues.
- To discover novel transcripts and genes related to reproductive traits.
Main Methods:
- RNA-sequencing (RNA-Seq) data from ovary, uterus, endometrium, pituitary, hypothalamus, liver, muscle, and adipose tissue.
- SNP discovery using pooled and individual sample approaches in Brangus cattle.
- Analysis of 1157 identified SNPs across five cattle breeds (Brangus, Brahman, Nellore, Angus, Holstein).
Main Results:
- 1157 SNPs were identified in 62 candidate genes.
- 172 SNPs within 13 genes were concordant across all five breeds.
- 12% of SNPs were located in exons (71% synonymous, 29% nonsynonymous), with others in introns or UTRs.
- 160 novel transcripts and five novel genes were identified.
Conclusions:
- The study identified numerous SNPs and novel genetic elements related to heifer puberty.
- Concordant SNPs across breeds offer potential targets for marker-assisted selection.
- Findings contribute to a deeper understanding of cattle reproductive genetics.
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