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Related Experiment Videos

Ten years of barcoding at the African Centre for DNA Barcoding.

B S Bezeng1, T J Davies1,2, B H Daru3

  • 1a African Centre for DNA Barcoding, University of Johannesburg, P.O. Box 524, Auckland Park 2006, Johannesburg, South Africa.

Genome
|March 25, 2017
PubMed
Summary

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The African Centre for DNA Barcoding has built a comprehensive DNA barcode library for southern African flora and fauna. This resource supports biodiversity research, aiding in ecological, evolutionary, and conservation studies.

Area of Science:

  • Biodiversity research
  • Molecular ecology
  • Systematics

Background:

  • The global initiative for DNA barcoding aims to rapidly survey biodiversity using short DNA sequences.
  • The mitochondrial cytochrome c oxidase 1 (CO1) gene is standard for animal barcoding.
  • Two plastid genes, rbcLa and matK, are recommended for terrestrial plant barcoding.

Purpose of the Study:

  • To establish a DNA barcode library for southern Africa.
  • To demonstrate the utility of DNA barcoding in ecological and evolutionary research.

Main Methods:

  • Utilized established animal (CO1) and plant (rbcLa, matK) DNA barcode markers.
  • Generated and contributed over 21,000 plant and 3,000 animal barcodes to the Barcode of Life Database (BOLD).
Keywords:
African flora and faunaCO1DNA barcodingapplications en écologiecodage à barres de l’ADNdéfinition d’espècesecological applicationsflore et faune africainesmatKrbcLaspecies delimitation

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Main Results:

  • Successfully created a substantial DNA barcode library for southern Africa.
  • Contributed significantly to the global Barcode of Life Database (BOLD).

Conclusions:

  • DNA barcoding is a powerful tool for biodiversity assessment.
  • The ACDB's work supports diverse applications in ecology, systematics, evolutionary biology, and conservation.