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Sample Preparation for Mass Cytometry Analysis

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Published on: April 29, 2017

Research Techniques Made Simple: Experimental Methodology for Single-Cell Mass Cytometry.

Tiago R Matos¹, Hongye Liu², Jerome Ritz²

¹Division of Hematologic Malignancies, Dana-Farber Cancer Institute, Boston, Massachusetts, USA; Harvard Medical School, Boston, Massachusetts, USA; Academic Medical Center, Department of Dermatology, University of Amsterdam, Amsterdam, The Netherlands.

The Journal of Investigative Dermatology

|March 26, 2017

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Mass cytometry by time-of-flight (CyTOF) offers single-cell resolution for over 40 markers, advancing cellular systems research. This review details CyTOF methods for accurate data acquisition in complex biological studies.

Area of Science:

Single-cell biology
Immunology
Biotechnology

Background:

Cellular systems exhibit complex interactions.
Mass cytometry (CyTOF) leverages time-of-flight mass spectrometry for high-resolution cellular analysis.
CyTOF enables detection of over 40 markers at the single-cell level with 135 detection channels.

Purpose of the Study:

To highlight the utility of CyTOF in investigative dermatology.
To demystify CyTOF techniques for broader application.
To review key experimental methods for accurate CyTOF data acquisition.

Main Methods:

Utilizes time-of-flight mass spectrometry.
Employs mass tags for ligand labeling.
Analyzes millions of cells for multiplexed marker detection.

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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

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Last Updated: Mar 5, 2026

Sample Preparation for Mass Cytometry Analysis

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Published on: April 29, 2017

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

Published on: November 2, 2012

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Published on: June 26, 2018

Main Results:

CyTOF allows deep characterization of cell populations, heterogeneity, and signaling pathways.
Potential applications include studying Langerhans cell differentiation and hair cycle signaling.
Methodological review focuses on optimizing signal detection and minimizing noise.

Conclusions:

CyTOF has profound potential to advance understanding of cellular heterogeneity and biological processes.
Accurate experimental methods are crucial for reliable CyTOF data and inter-sample comparisons.
This review provides essential guidance for implementing CyTOF in research, particularly in dermatology.