A new and efficient Solid Phase Microextraction approach for analysis of high fat content food samples using a
Selenia De Grazia1, Emanuela Gionfriddo2, Janusz Pawliszyn2
1Department of Chemistry, University of Waterloo, Ontario N2L 3G1, Canada; Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute (SCIFAR), University of Messina, Viale Annunziata, 98168 Messina, Italy.
Talanta
|March 26, 2017
Summary
A new Solid Phase Microextraction (SPME) coating enhances avocado sample analysis by minimizing matrix fouling. The PDMS/DVB/PDMS coating offers improved durability for contaminant quantification in fatty matrices.
Area of Science:
- Analytical Chemistry
- Environmental Science
- Food Science
Background:
- Direct extraction of avocado samples using Solid Phase Microextraction (SPME) requires robust coatings resistant to matrix fouling.
- Optimizing washing steps is crucial for extending the lifetime of SPME coatings in complex matrices like avocado.
- Existing coatings may suffer from irreversible matrix attachment, reducing extraction efficiency over successive analyses.
Purpose of the Study:
- To optimize a protocol for direct avocado sample extraction using a novel, matrix-compatible SPME coating.
- To enhance the lifetime and performance of SPME coatings through optimized pre-desorption and post-desorption washing steps.
- To compare the durability and efficiency of a commercial PDMS/DVB coating with a new PDMS/DVB/PDMS coating for avocado analysis.
Main Methods:
- Optimization of pre-desorption and post-desorption washing steps (solvent type, time, agitation).
- Evaluation of SPME coating lifetime over 80 successive extractions using PDMS/DVB and PDMS/DVB/PDMS coatings.
- Analysis of matrix component attachment and desorption artifacts using Gas Chromatography-Gas Chromatography-Time of Flight Mass Spectrometry (GCxGC-ToF/MS).
Main Results:
- The PDMS/DVB/PDMS coating demonstrated enhanced inertness towards matrix fouling compared to the standard PDMS/DVB coating.
- The PDMS/DVB coating showed reduced extractive performance after 80 extractions due to irreversible matrix attachment.
- Optimized washing steps contributed to the extended lifetime and consistent performance of the new SPME coating.
Conclusions:
- The PDMS/DVB/PDMS coating represents a significant advancement for robust SPME methods in fatty matrices like avocado.
- Minimal sample pre-treatment is achievable with the developed SPME protocol, streamlining contaminant quantification.
- Understanding matrix component interactions with SPME coatings is vital for developing reliable analytical methods.
