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Expression Profile of MicroRNA Biogenesis Components in Renal Transplant Patients
E Celen1, M G Ertosun1, H Kocak2
1Department of Medical Biology and Genetics, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
Transplantation Proceedings
|March 26, 2017
Summary
MicroRNA (miRNA) biogenesis components in kidney transplant patients decrease post-transplant, with unique changes linked to immunosuppression and rejection. These components may serve as biomarkers for graft outcomes.
Area of Science:
- Nephrology
- Molecular Biology
- Immunology
Background:
- MicroRNAs (miRNAs) and their biogenesis pathways are implicated as biomarkers in diseases like cancer and diabetes.
- Investigating miRNA biogenesis components in renal transplantation is crucial for understanding post-transplant health.
Purpose of the Study:
- To examine miRNA biogenesis component expression in renal transplant recipients before and after transplantation.
- To correlate these expression profiles with immunosuppressive therapy and clinical outcomes.
Main Methods:
- Gene and protein expression of Dicer, Drosha, Pasha (DGCR8), Exportin5 (XPO5), and Argonaute2 (AGO2) were analyzed in PBMCs.
- Real-time quantitative PCR and Western blot were used.
- First-time renal transplant recipients were studied before and 3 months post-transplant.
Main Results:
- Gene expression of miRNA biogenesis components significantly decreased post-transplantation.
- Patients with chronic rejection showed a higher reduction rate.
- Everolimus treatment affected Dicer and AGO2 gene expression.
- Protein expression of Dicer, Drosha, DGCR8, and AGO2 decreased, while XPO5 remained unchanged in non-rejecting patients.
Conclusions:
- Immunosuppressive treatment globally impacts the miRNA biogenesis pathway.
- miRNA biogenesis components show potential as biomarkers for kidney graft outcomes.
- These components may represent viable pharmacologic targets.
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