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Curing of endogenous 2 micron DNA in yeast by recombinant vectors
1Molecular Genetics Department, Smith Kline-RIT, Rixensart, Belgium.
Current Genetics
|January 1, 1987
Abstract:
Passaging on selective media of yeast strains transformed with complete 2 micron vectors carrying TRP1, LEU2 or URA3 selective markers leads to curing of the resident endogenous 2 micron DNA in a majority of the population. Vector plasmids defective in FLP function are fixed as populations of A, A + B or B forms after 2 micron loss. Transformation with these plasmids offers a general method of obtaining cir degree derivatives of any yeast strain.