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Phosphorylation of D-glyceraldehyde-3-phosphate dehydrogenase by Ca2+/calmodulin-dependent protein kinase II
L I Ashmarina1, S E Louzenko, S E Severin
1A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
FEBS Letters
|April 25, 1988
Abstract:
Rabbit muscle D-glyceraldehyde-3-phosphate dehydrogenase was shown to serve as a substrate for Ca2+/calmodulin-dependent protein kinase II with a Km of 0.33 microM and a Vmax of 2.63 mumol.min-1.mg-1 at pH 7.5 and 30 degrees C. In the absence of calmodulin, the Vmax was halved and Km unchanged. 0.99 mol of phosphate was incorporated per tetrameric molecule of D-glyceraldehyde-3-phosphate dehydrogenase under the experimental conditions employed.