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The Drosophila ets-2 gene: molecular structure, chromosomal localization, and developmental expression
L J Pribyl1, D K Watson, M J McWilliams
1Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21701-1013.
Developmental Biology
|May 1, 1988
Summary
Researchers identified a highly conserved Drosophila ets-2 gene, an oncogene homolog, showing over 90% amino acid homology to its viral counterpart. This discovery highlights the utility of Drosophila as a model for studying ets gene function.
Area of Science:
- Molecular Biology
- Developmental Biology
- Genetics
Background:
- The ets gene family, originating from the avian erythroblastosis retrovirus E26, has cellular homologs studied across various species, including chickens, humans, mice, and cats.
- Investigating these homologs aids in understanding cellular transformation and oncogenesis.
Purpose of the Study:
- To isolate and characterize a Drosophila homolog of the ets gene, extending evolutionary studies.
- To analyze the sequence, conservation, expression patterns, and chromosomal location of this Drosophila ets-related gene.
Main Methods:
- Genomic cloning and sequence analysis of the Drosophila ets-related gene.
- Comparison of predicted amino acid sequences with known v-ets sequences.
- Analysis of gene expression throughout Drosophila development.
- In situ hybridization to determine chromosomal localization.
Main Results:
- A Drosophila ets-related genomic clone, designated ets-2, was isolated and characterized.
- The ets-2 clone contains the 3' end of the v-ets gene, sharing over 90% amino acid homology with v-ets.
- This represents the highest conservation level for any known Drosophila oncogene homolog.
- ets-2 is expressed throughout development, with peak expression during embryonic and pupal stages.
- The ets-2 gene is mapped to chromosomal position 58A/B, with no known associated phenotypic mutants.
Conclusions:
- The high degree of conservation suggests a critical role for the ets-2 gene.
- The Drosophila model system offers a valuable platform for future functional studies of the ets gene due to its conserved nature and amenability to genetic manipulation.