Jove
Visualize
Contact Us

Related Experiment Videos

Tn7 transposition: two transposition pathways directed by five Tn7-encoded genes.

C S Waddell1, N L Craig

  • 1Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0552.

Genes & Development
|February 1, 1988
PubMed
Summary

The bacterial transposon Tn7 uses five essential genes for transposition. Two distinct pathways, controlled by tnsD and tnsE genes, mediate transposition to specific or other sites in E. coli.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tn7: smarter than we thought.

Nature reviews. Molecular cell biology·2001
Same author

Selective recognition of pyrimidine motif triplexes by a protein encoded by the bacterial transposon Tn7.

Journal of molecular biology·2001
Same author

Tn7 recognizes transposition target structures associated with DNA replication using the DNA-binding protein TnsE.

Genes & development·2001
Same author

Target DNA structure plays a critical role in Tn7 transposition.

The EMBO journal·2001
Same author

Analysis of gain-of-function mutants of an ATP-dependent regulator of Tn7 transposition.

Journal of molecular biology·2001
Same author

The rib1 mutant is resistant to indole-3-butyric acid, an endogenous auxin in Arabidopsis.

Plant physiology·2000
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Bacterial genetics
  • Molecular biology
  • Transposable elements

Background:

  • The bacterial transposon Tn7 exhibits site-specific transposition to attTn7 and non-specific transposition.
  • Understanding the genetic basis of Tn7 transposition is crucial for comprehending its mobility and regulatory mechanisms.

Purpose of the Study:

  • To identify and characterize genes essential for Tn7 transposition.
  • To elucidate the distinct mechanisms underlying Tn7's site-specific and non-specific transposition pathways.

Main Methods:

  • In vitro insertional mutagenesis was employed to identify essential Tn7 transposition genes.
  • Characterization of the identified genes' roles in different transposition events.

Main Results:

Related Experiment Videos

  • Five essential Tn7 transposition (tns) genes were identified: tnsA, tnsB, tnsC, tnsD, and tnsE.
  • Genes tnsA, tnsB, and tnsC are necessary but not sufficient for all Tn7 transposition.
  • TnsD specifically mediates transposition to the attTn7 site.
  • TnsE specifically mediates transposition to non-attTn7 sites.

Conclusions:

  • Tn7 transposition is regulated by at least five genes.
  • Tn7 possesses two distinct, yet overlapping, transposition pathways.
  • The tnsD and tnsE genes play critical, pathway-specific roles in Tn7 transposition.