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Mesophilic continuous fermentative hydrogen production from acid pretreated de-oiled jatropha waste hydrolysate using
Gopalakrishnan Kumar1, Periyasamy Sivagurunathan2, Biswarup Sen3
1Sustainable Management of Natural Resources and Environment Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Vietnam.
Abstract:
Mesophilic hydrogen production from acid pretreated hydrolysate (biomass concentration of 100g/L and 2% hydrochloric acid) of de-oiled jatropha waste was carried out in continuous system using immobilized microorganisms at various hydraulic retention times (HRTs) ranging from 48 to 12h. The experimental results of the reusability of immobilized microorganisms showed their stability up to 10 cycles with an average cumulative hydrogen production of 770mL/L. The peak hydrogen production rate and hydrogen yield were 0.9L/L*d and 86mL/greducing sugars added, respectively at 16h HRT, with butyrate as the predominant volatile fatty acid. The microbial community analysis revealed that majority of the PCR-DGGE bands were assigned to genus Clostridium and were perhaps the key drivers of the higher hydrogen production.
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