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Related Experiment Video

Updated: Mar 5, 2026

Expanding the Toolkit for In Vivo Imaging of Axonal Transport
09:24

Expanding the Toolkit for In Vivo Imaging of Axonal Transport

Published on: December 23, 2021

4.1K

Methodological advances in imaging intravital axonal transport.

James N Sleigh1, Alessio Vagnoni2, Alison E Twelvetrees1

  • 1Sobell Department of Motor Neuroscience and Movement Disorders, Institute of Neurology, University College London, London, UK.

F1000Research
|March 28, 2017
PubMed
Summary

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Axonal transport, essential for neuron survival, is impaired by aging and neurodegenerative diseases. New intravital imaging techniques offer real-time insights into this vital process in living organisms.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biophysics

Background:

  • Axonal transport facilitates the movement of essential cargoes within neurons, crucial for their function and survival.
  • This bidirectional process is vital for neuronal health but is compromised in aging and neurodegenerative conditions like Alzheimer's disease and ALS.
  • Current in vitro studies of axonal transport may not fully replicate in vivo conditions.

Purpose of the Study:

  • To review and highlight recent advances in intravital imaging techniques for monitoring axonal transport.
  • To discuss the strengths and limitations of these novel methodologies.
  • To provide insights into the dynamic process of axonal transport in vivo.

Main Methods:

  • Adaptation of intravital imaging techniques originally developed for other biological processes.
Keywords:
axonal transportintravital imagingneurons

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Last Updated: Mar 5, 2026

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  • Real-time monitoring of axonal transport in live organisms.
  • Comparative analysis of in vitro and in vivo axonal transport studies.

    • Main Results:

    • Intravital imaging provides novel, real-time insights into axonal transport dynamics.
    • These advanced techniques offer a more accurate representation of in vivo axonal transport compared to traditional in vitro methods.
    • Methodological strengths and limitations are identified, paving the way for future improvements.

    Conclusions:

    • Intravital imaging represents a significant methodological advance in studying axonal transport.
    • These techniques are crucial for understanding how axonal transport functions and is affected by disease and aging in vivo.
    • Further research and refinement of these methods are needed to fully elucidate the complexities of axonal transport.