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Updated: Mar 5, 2026
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: March 8, 2020
Quantitative analysis of lipids: a higher-throughput LC-MS/MS-based method and its comparison to ELISA
Adarsh S Gandhi1, David Budac1, Tanzilya Khayrullina2
1Molecular Pharmacology, Bioanalysis & Operations, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA; Molecular Pharmacology, Bioanalysis & Operations, Lundbeck Research USA, 215 College Road, Paramus, NJ, USA.
Aim:
Lipids such as prostaglandins, leukotrienes and thromboxanes are released as a result of an inflammatory episode in pain (central and peripheral).
Methodology & Results:
To measure these lipids as potential mechanistic biomarkers in neuropathic pain models, we developed a higher-throughput LC-MS/MS-based method with simultaneous detection of PGE2, PGD2, PGF2α, LTB4, TXB2 and 2-arachidonoyl glycerol in brain and spinal cord tissues. We also demonstrate that the LC-MS/MS method was more sensitive and specific in differentiating PGE2 levels in CNS tissues compared with ELISA.
Conclusion:
The ability to modify the LC-MS/MS method to accommodate numerous other lipids in one analysis, demonstrates that the presented method offers a cost-effective and more sensitive alternative to ELISA method useful in drug discovery settings.
