Exploring and validating physicochemical properties of mangiferin through GastroPlus^® software

Area of Science:

• Pharmacology
• Drug Delivery
• Physical Chemistry

Background:

• Mangiferin (Mgf) is a therapeutic polyphenol with limited oral absorption.
• Effective delivery systems are crucial for enhancing Mgf's gastrointestinal uptake.
• Detailed physicochemical data for Mgf is lacking in existing literature.

Purpose of the Study:

• To elucidate the physicochemical characteristics of Mangiferin (Mgf).
• To provide essential data for the development of novel Mgf delivery systems.
• To utilize GastroPlus™ software for in-silico analysis of Mgf properties.

Main Methods:

• Physicochemical properties including aqueous solubility, log P, and P_eff were determined.
• Mangiferin's interaction with P-glycoprotein (P-gp) was assessed using Caco-2 cell assays.
• In-situ intestinal perfusion studies were conducted to estimate effective permeability (P_eff).
• Characterization involved techniques like TEM, DSC, IR spectroscopy, and powder XRD.

Main Results:

• Aqueous solubility was found to be 0.38 mg/ml.
• Log P value was determined to be -0.65.
• Effective permeability (P_eff) was measured at 0.16 × 10^-4 cm/s.
• Mangiferin was identified as a P-gp substrate.

Conclusions:

• The reported physicochemical properties of Mangiferin are vital for designing advanced drug delivery systems.
• This data will guide researchers in overcoming Mgf's bioavailability challenges.
• The study provides a foundation for future Mangiferin formulation development.