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Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
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Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

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Apoptotic pathways of macrophages within osteolytic interface membrane in periprosthestic osteolysis after total hip

Guoyin Liu1,2, Ting Guo1, Yong Zhang2

  • 1Department of Orthopaedics, Jinling Hospital affiliated to School of Medicine, Nanjing University and State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, China.

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
|March 28, 2017
PubMed

Insights

Wear particles trigger macrophage apoptosis via endoplasmic reticulum stress, mitochondrial dysfunction, and death receptor pathways, worsening osteolysis. Targeting these pathways may prevent periprosthetic osteolysis.

Area of Science:

  • Biomedical Engineering
  • Immunology
  • Orthopedic Surgery

Background:

  • Macrophage apoptosis is implicated in osteolysis, but the underlying mechanisms and pathway correlations remain unclear.
  • Understanding macrophage apoptosis pathways is crucial for addressing periprosthetic osteolysis progression.

Purpose of the Study:

  • To elucidate the apoptotic mechanisms of macrophages in osteolysis.
  • To investigate the relationship between apoptotic pathways and osteolysis progression.

Main Methods:

  • Transmission electron microscopy (TEM) for wear particle ultrastructure analysis.
  • TUNEL staining for in situ apoptotic macrophage identification.
  • Immunohistochemistry and Western blotting for apoptotic biomarker expression analysis.

Main Results:

  • Wear particles in the 30-60 nm range were prevalent in osteolytic interface membranes.
  • Macrophage apoptosis ratio correlated positively with osteolysis progression.
  • Elevated biomarkers for ER stress, mitochondrial dysfunction, and death receptor pathways were observed in osteolytic tissues.

Conclusions:

  • Implant-generated wear particles accelerate macrophage apoptosis via interconnected ER stress, mitochondrial, and death receptor pathways.
  • These apoptotic pathways are significantly altered in periprosthetic osteolysis.
  • Targeting these specific macrophage apoptotic pathways offers potential for preventing and treating periprosthetic osteolysis.
Keywords:
Osteolysisapoptosisdeath receptor pathwayendoplasmic reticulum stress pathwaymacrophagesmitochondrion pathway

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