Native Mass Spectrometry of Recombinant Proteins from Crude Cell Lysates
Jinrui Gan1, Gili Ben-Nissan1, Galina Arkind1
1Department of Biomolecular Sciences, Weizmann Institute of Science , Rehovot 7610001, Israel.
Analytical Chemistry
|March 28, 2017
Abstract:
Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, oligomeric state, overall structure, and stability, as well as ligand binding, without the need for purification.


